Informatics Publishing Limited

S. Lakshmi ¹, R. Siva Kumar ², S. Rajendran ³

Affiliations
1 Jeppiaar Engineering College, Chennai, IN
2 Barathiar University, IN
3 Department of Chemistry, R. V. S. School of Engineering and Technology, Dindigul-5, IN

Abstract

The blue green microalgae Spirulina platensis is an important source of nutrients. An important obstacle for the production of Spirulina is Rotifer. In this paper we present a method for automated identification and recognition of rotifer contamination in Spirulina using image processing techniques. Getting the expert's advice is an essential one to improve the production. Our primary objective is to provide high nutrients Spirulina free from contaminations and secondary objective is to solve the quality issue by identifying rotifer contaminations at the earliest and giving suggestions through the mobile phones. Using microscopic examination for identifying rotifier in live culture is time consuming and manmade process. There is a chance for man made error and our new technique is machine made and the errors to be minimized. Existing system need qualified persons for operation and our proposed system can be operated by anyone. We examine lab scale Spirulina culture through both existing and new techniques for recognizing the rotifer. Our proposed tool for identifying contaminations at beginning stage is easy to eradicate the contaminations from live culture. It will be helpful to biologist to take necessary actions against contaminations of the same before further multiplications of contaminations. According to the Survey of Sun food Super Food, in 2020 the expected world wide Spirulina production is about 220 thousand tons. Hence Spirulina can be a solution for solving the world nutrition problems due to its very high growth rate and high nutritional value. Our research-automatic identification of contamination in algae through the processing of mobile phone images is an easiest way for improving and evaluating the growth of Spirulina for effective cultivation of algae.

Keywords

Automatic Detection and Contamination, Image Processing, Recognition, Rotifer, Spirulina.

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R. Siva Kumar , P. Senthil Kumaran , P. Kumar Nallasivan , W. D. Sam Solomon , R. Venkatnarayanan

Abstract

A new simple, accurate, precise and reproducible Reverse Phase-High Performance Liquid Chromatographic method has been developed for the simultaneous estimation of Aceclofenac and Tizanidine in tablet dosage forms using C₁₈ column (Ineretsil, 250 x 4.6 mm, 5 μm) in isocratic mode. The mobile phase consisted of acetonitrile, methanol and 20 mM phosphate buffer adjusted to pH 3.5 in ratio of (40:30:30 v/v) with Ultraviolet-Visible detection at 230 nm. The method was linear over the concentration range for aceclofenac120-280 μg/ml and for tizanidine 2-40 μg/ml. The recoveries of Tizanidine and Aceclofenac were found to be in the range of 99.45-100.61% and 99.56-101.32% respectively. The validation of method was carried out using International Conference on Harmonization-guidelines. The described High Performance Liquid Chromatographic method was successfully employed for the analysis of pharmaceutical formulations containing combined dosage form.

Keywords

Simultaneous Estimation, RP - HPLC, Aceclofenac, Tizanidine, ICH Guidelines.

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T. Akelesh , R. Siva Kumar , R. Jothi , Vijairajan , P. Arulraj , R. Venkatnarayanan

Abstract

The aim of the present work is to analyze the standards of marketed cosmetic products which are largely consumed in day to day life of the people. The cosmeceutical should be tested for efficacy to ensure a proven skin benefit and also to substantiate marketing claims. The work was done by keeping the ideas of Bureau of Indian Standards to analyze the cosmetic products. The evaluation for the following cosmetics such as tooth pastes (Colgate, Closeup, Pepsodent, Vicco and Anchor) and face powders (Ponds, Eva, Fa, Cuticura and Spinz) are performed for their quality. All the marketed tooth pastes and face powders which had been evaluated complied with the standards specified by Bureau of Indian Standards. Hence all the selected marketed tooth pastes and selected face powders were found to be of good quality.

Keywords

Cosmetic Products, Bureau of Indian Standards, Tooth Paste, Face Powder.

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J. Anu Deepa ¹, N. Santhi ¹, R. Ramya ¹, R. Siva Kumar ¹, R. Venkata Narayanan ¹

Affiliations
1 R.V.S College of Pharmaceutical Sciences, Sulur, Coimbatore – 641 402, IN

Abstract

A new simple, sensitive and precise visible spectrophotometric method has been developed for the determination of Secnidazole in bulk and in pharmaceutical formulation. This method is based on the reduction of secnidazole, in the presence of 4N Hydrochloric acid and ethanol to form yellow colored chromogen. Which was estimated at an absorption maximum of 477.5 nm. This method has the linearity in the concentration range of 100-500μg/ml. This method is statistically evaluated for efficacy.

Keywords

Secnidazole, Reduction Method.

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C. S. Kandasamy ¹, Ch. Sai Lohit ², R. Siva Kumar ², V. Gopal ³, Chandini ², R. Nair ⁴, R. Venkatanarayanan ²

Affiliations
1 Department of Pharmacognosy, R.V.S College of Pharmaceutical Sciences, Sulur, Coimbatore – 641 402, Tamil Nadu, IN
2 Department of Pharmacognosy, R.V.S College of Pharmaceutical Sciences, Sulur, Coimbatore, IN
3 Department of Pharmacognosy, College of Pharmacy, Mother Theresa Institute of Health Sciences and Research Institute, Pondicherry, IN
4 Department of Biochemistry, SASTRA University, Tanjore, IN

Abstract

Extracts of crude drugs such as Ajowan, Mace, Cardamom, Cumin, Nutmeg and Clove used in the Polyherbal formulation - RVSPHF567 were taken in account and dissolved in their appropriate solvents. These extracts were analyzed individually by High Performance Thin Layer Chromatography (HPTLC) to detect the presence of their phytoconstituents. The instrument used for High Performance Thin Layer Chromatography (HPTLC) was CAMAG LINOMAT 5. These analytical investigations were highly useful for preparing Polyherbal formulation - RVSPHF567 which is highly effective as Hepatoprotective agent and can be used in-vivo as a potential therapeutic agent. The sample was prepared by solvent extraction. Then 2μl of test solution was loaded as 6mm band length on the 9 x 10 Silica gel 60F254 TLC plate. This was done by using Hamilton syringe. These plates were kept in respective mobile phase Chloroform: Methanol: Water (6.5: 2.5: 0.4) for spot development to 90mm. These plates were dried and kept in Photo-documentation chamber - CAMAG REPROSTAR 3. The images were captured at White light, UV 254nm and UV 366nm. The developed plates were sprayed with respective spray reagent and photo-documented in White light and UV 366nm mode. Then the same were scanned at 500nm. The Peak table, Peak display and Peak Densitogram show the terpenoids profile in all the crude drugs.

Keywords

Polyherbal, Liver Protective.

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R. Siva Kumar ¹, Shaik Nafeez Basha ¹, P. Kumar Nallasivan ¹, W. D. Sam Solomon ¹, R. Venkatnarayanan ¹

Affiliations
1 Dept. of Pharmaceutical Chemistry, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, (T.N.), IN

Abstract

The Protein-Ligand interaction plays a significant role in structural based drug designing. The highly pathogenic influenza A virus subtype H5N1 virus is an emerging avian influenza virus that has been causing global concern as a potential pandemic threat. It is often referred to simply as "bird flu" or "avian influenza". In our research work we have taken influenza A virus H5N1 receptor. The receptor was docked to the commercially available drugs zanamivir and oseltamivir and the energy value obtained are as follows; zanamivir (-231.74) and oseltamivir (-243.74) using the HEX docking software. We tried to improve the binding efficiency and steric compatibility of zanamivir against N5N1 receptor. Several modifications were made to the probable functional groups which were interacting with the receptor molecule. Analogs of this drug molecule were prepared using ACD ChemSketch and docked using HeX docking software. Zanamivir analog 3 and oseltamivir analog 5 were detected with significant energy values and probable lead molecules. The Modified drugs was sketched using Chemsketch were found to be better than the conventional drugs available. Further from this work we can improve the steric compatibility and then ADME properties of the Analogs can be analyzed using Inslico ADME tools available.

Keywords

Bird Flu, Chemsketch, Docking, Hex, Rasmol.

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R. Siva Kumar ¹, P. Kumar Nallasivan ¹, S. Saravanakumar ¹, C. S. Kandasamy ¹, R. Venkatnarayanan ¹

Affiliations
1 Dept. of Pharmaceutical Analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641402, Tamilnadu, IN

Abstract

Reverse Phase HPLC method for the determination of nitazoxanide and ofloxacin in bulk and tablet formulations. The determination was carried out by using Phenomenex C18 column with 0.24% sodium lauryl sulphate:acetonitrile:acetic acid (pH-4.0) (58:40:02) as the mobile phase. The flow rate was 1.5 ml/min and the eluent was monitored at 295 nm. The Retention time of nitazoxanide and ofloxacin were 2.2 and 5.4 respectively. Linearity for the nitazoxanide and ofloxacin were found in the range of 400-600 μg/ml and 160-240 μg/ml respectively. The method was reproducible, with good resolution between nitazoxanide and ofloxacin and can be use for routine analysis.

Keywords

RP-HPLC, Nitazoxanide and Ofloxacin.

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